Search results for " STR typing"

showing 1 items of 1 documents

The molecular characterization of a depurinated trial DNA sample can be a model to understand the reliability of the results in forensic genetics

2014

The role of DNA damage in PCR processivity/fidelity is a relevant topic in molecular investigation of aged/forensic samples. In order to reproduce one of the most common lesions occurring in postmortem tissues, a new protocol based on aqueous hydrolysis of the DNA was developed in vitro. Twenty-five forensic laboratories were then provided with 3.0 μg of a trial sample (TS) exhibiting, in mean, the loss of 1 base of 20, and a molecular weight below 300 bp. Each participating laboratory could freely choose any combination of methods, leading to the quantification and to the definition of the STR profile of the TS, through the documentation of each step of the analytical approaches selected. …

DNA depurination; Forensic genetics; PCR fidelity; STR typing; Biochemistry; Clinical BiochemistryPCR fidelityGenotyping TechniquesDNA damageSample (material)Clinical BiochemistryDNA depurinationReproducibility of ResultForensic geneticsBiologyPolymerase Chain ReactionBiochemistryNOAnalytical Chemistrylaw.inventionDNA depurination; PCR fidelity; STR typing; forensic genetics.Settore MED/43 - Medicina LegalelawSettore BIO/13 - Biologia ApplicataGenotypeHumansSTR typingGenotyping TechniquesPolymerase chain reactionProtocol (science)GeneticsMedicine (all)Reproducibility of ResultsForensic geneticDNAAmpliconDNA FingerprintingDNA depurination; Forensic genetics; PCR fidelity; STR typingSettore BIO/18 - GeneticaDNA depurination Forensic genetics PCR fidelity STR typingDNA profilingSettore MED/03 - Genetica MedicaMicrosatellite RepeatGenotyping TechniqueDNA depurination; Forensic genetics; PCR fidelity; STR typing;Microsatellite RepeatsHuman
researchProduct